Which procedure is NOT required by AABB Standards in the investigation of a suspected hemolytic transfusion reaction?

The investigation of a suspected hemolytic transfusion reaction involves a series of critical steps to ensure patient safety and identify any potential issues. Among these, the procedure that is not mandated by AABB Standards is the repetition of the antibody screen on the pretransfusion sample.

In the context of a suspected transfusion reaction, the primary focus is on confirming the identity of the blood units and the patient's own blood type, which is addressed through checking labels and comparing serum samples. The necessity to repeat the ABO typing on the posttransfusion sample helps verify whether there was any discrepancy or error that occurred during the transfusion process. Comparing pretransfusion and posttransfusion serum or plasma helps track any changes caused by the transfusion.

In contrast, repeating the antibody screen on the pretransfusion sample does not provide additional value in the investigation of an acute reaction, as the initial screen has already been performed prior to transfusion. When a suspected reaction is assessed, the focus is more on posttransfusion samples and the units involved rather than retesting the antibody screen that was initially done. Therefore, this procedure is not a requirement according to AABB Standards during the investigation of a suspected hemolytic transfusion reaction.